Tuesday, December 17, 2013

1.2.1 Detection of proteins Popular detection legionella of viral proteins that are highly antigeni


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a. Indirect enzyme immunoassay principle is plastic coated with entity beans Jane then put the serum to react. Then react with antihuman gamma globulin legionella and chromogenic sustrate see color changes legionella occurred compared to control if the value is higher than cut off considered legionella positive (reactive).
b. Competitive enzyme immunoassay principle is plastic coated with entity beans Jane then put the serum to react. With the addition of antibodies against HIV wash the non-reactive, then fill chromogenic subtrate see color changes that occur and calculate the cut off compared to control if the value is higher than cut off considered legionella positive (. reactive)
c. Antigen double sandwich immunoassay principle is coated seeds with plastic entity Jane then put the serum to react with additives antigens clear that no reaction is then filled chromogenic substrate legionella see color changes occurred compared to control if the. higher than cut off considered positive (reactive).
HIV rapidly, within 30 minutes, and it's quite simple, do not require special tools or equipment can read without glasses. In principle, the reaction of antigens with antibodies targeting the procedure varies depending on the container used. Containers used today are three types of cartridge and slide the strip.
1.2.1 Detection of proteins Popular detection legionella of viral proteins that are highly antigenic capsid or core protein is p24, a viral cover. Three detectable in the bloodstream and detected by other proteins in patients infected with HIV in the acute phase. Using an ELISA principle.
Right lymphatic Science A method as simple and cheap with high sensitivity and specificity. Can be graded according to the different strategies (Strategy I, II, III) recommended by the World Health Organization legionella and UNAIDS to the year 2540 (1997), which relies on the prevalence of HIV infection in the population. Check the Thus able to report positive results, negative results without confirming with the West Terminal legionella Block Group (Western blot), however, the West Terminal Block Group. Remains the standard method (Gold standard) to confirm infection. In the event of conflicting results after. By various strategies
From the prevalence of HIV infection in the general population in 2545 stood at 1.8 percent, legionella so that the antibodies to HIV, the national Ministry of Health. Recommended for use with the third set of tests in a test suite tactically 3 (Strategy III) as recommended by the World Health Organization and UNAIDS.
Proficiency testing to detect antibodies to HIV, the agency that provides proficiency testing. legionella And quality control tests, as appropriate, at least 2-3 times a year to select the method of viral components. To diagnose infection in children.
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